RNAlater®
SIGMA/R0901 - Stabilize and protect RNA with immediate RNase inactivation
Synonym: RNA preservation solution; RNA protection solution; RNA protector; RNA stabilizer; RNA storage solution; RNA transportation solution
MDL Number: MFCD03453003
Product Type: Chemical
Superior Protection Against RNAse Degradation
A 5 mL aliquot of RNase A (Cat. No. R6513; serially diluted to final concentrations of 4.5×10-5 – 4.5×10-11 units/μL) was added to 5 mg RNA (Cat. No. R7020) in 15 μL containing either 10 μL of RNAlater (top panel) or TE buffer (bottom panel). Reactions were incubated at 37 °C for 20 minutes, purified using the GenElute™ Total RNA kit (Cat. No. RTN10) and analyzed on a 1% agarose gel.
A 5 mL aliquot of RNase A (Cat. No. R6513; serially diluted to final concentrations of 4.5×10-5 – 4.5×10-11 units/μL) was added to 5 mg RNA (Cat. No. R7020) in 15 μL containing either 10 μL of RNAlater (top panel) or TE buffer (bottom panel). Reactions were incubated at 37 °C for 20 minutes, purified using the GenElute™ Total RNA kit (Cat. No. RTN10) and analyzed on a 1% agarose gel.
Stabilizes Samples at Room Temperature for Up to One Week
Quality of RNA isolated from tissue stored in RNAlater solution. Fresh mouse tissues were dissected and stored in RNAlater at 37 °C for 1 day, room temperature for 1 week, or 4 °C for 1 month. RNA was isolated using TRI Reagent (Cat. No. T9424) and analyzed using denaturing agarose gel electrophoresis.
Quality of RNA isolated from tissue stored in RNAlater solution. Fresh mouse tissues were dissected and stored in RNAlater at 37 °C for 1 day, room temperature for 1 week, or 4 °C for 1 month. RNA was isolated using TRI Reagent (Cat. No. T9424) and analyzed using denaturing agarose gel electrophoresis.
| InChI | 1S/C37H67N15O13/c1-16(2)1 |
| InChI key | DUCQRBNNHJNRMP-KDDVYCELSA |
| Quality Level | 100  |
| SMILES string | N([C@@H]([C@H](O)C)C(=O)N |
| Application: | RNAlater® has been used to synthesize antisense RNA probe. It can be used with various downstream applications including mRNA and total RNA isolation, histology and immunocytochemistry and is compatible with Sigma′s GenElute™ isolation kits. |
| Biochem/physiol Actions: | RNAlater is easy to use. Simply cut tissue samples to be stored so they are less than 0.5 cm in at least one dimension and submerge in 5 volumes of RNAlater. Small organs, such as rat kidney, liver or spleen can be stored in whole in RNAlater. When ready to isolate the RNA, remove the tissue from RNAlater and process as though just harvested. For cell storage, resuspend pelleted cells in a small amount of PBS before adding 5-10 volumes of RNAlater. Before preparing RNA, pellet cells and discard supernatant. |
| Features and Benefits: | • No compromise in RNA quality following mRNA or total RNA isolation • Rapidly permeates tissues to stabilize and protect cellular RNA • Aqueous non-toxic solution allows downstream tissue processing • Stabilizes samples at room temperature |
| General description: | RNAlater can be used with various downstream applications including mRNA and total RNA isolation, histology and immunocytochemistry and is compatible with Sigma′s isolation kits. |
| General description: | RNAlater is compatible with most RNA isolation methods, including TRI Reagent and GenElute Total RNA isolation and mammalian mRNA isolation kits. RNAlater is an aqueous, non-toxic tissue storage reagent that rapidly permeates tissue to stabilize and protect cellular RNA in situ in unfrozen specimens. Tissue pieces are harvested and immediately submerged in RNAlater for storage without jeopardizing the quality or quantity of RNA. RNAlater eliminates the need to immediately process tissue specimens or to freeze samples in liquid nitrogen for later processing. RNAlater preserves RNA in tissues for up to 1 day at 37 °C, 1 week at 25 °C, and 1 month at 4 °C. Tissues can also be stored at -20 °C long-term. RNAlater has been extensively tested on several tissues from vertebrate species, including brain, heart, kidney, spleen, liver, testis, skeletal muscle, fat, lung, and thymus. RNAlater is also effective for E. coli, Drosophila, tissue culture cells, white blood cells, and some plants. |
| Legal Information: | GenElute is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | Licensed for distribution by Ambion, Inc., The RNA Company. |
| Legal Information: | RNAlater is a registered trademark of Ambion, Inc. |
| Other Notes: | RNAlater® is an aqueous, non-toxic tissue and cell storage reagent that stabilizes and protects cellular RNA in intact, unfrozen tissue and cell samples. RNAlater eliminates the need to immediately process samples or to freeze samples in liquid nitrogen for later processing. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12352200 |