Ribonuclease T1 from Aspergillus oryzae
SIGMA/R1003 - ammonium sulfate suspension, 300,000-600,000 units/mg protein
Synonym: Guanyloribonuclease; Ribonucleate 3′-guanylo-oligonucleotidohydrolase
CAS Number: 9026-12-4
EC Number: 232-809-1
MDL Number: MFCD00132191
Product Type: Chemical
Catalyzes the two-stage endonucleolytic cleavage of RNA to yield nucleoside 3′-phosphates and 3′-phosphooligonucleotides ending mainly in Gp. In the reaction, cleavage occurs between the 3′-phosphate group of a guanidine ribonucleotide and 5′-hydroxyl of the adjacent nucleotide. The initial product is a 2′:3′cyclic phosphate nucleoside that is hydrolyzed to the corresponding 3′-nucleoside phosphate.
| application(s) | cell analysis |
| biological source | Aspergillus sp. ( |
| form | ammonium sulfate suspension |
| mol wt | 11068 by amino acid sequence |
| Quality Level | 200  |
| specific activity | 300,000-600,000 units/mg protein |
| storage temp. | 2-8°C |
| suitability | suitable for separating native or denatured proteins, or nucleic acids |
| technique(s) | cell based assay: suitable |
| Analysis Note: | Protein determined by E |
| Application: | Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies . |
| Biochem/physiol Actions: | Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues. Cleavage occurs between the 3’-phosphate group of a guanidine ribonucleotide and 5’-hydroxyl of the adjacent nucleotide. The initial product is a 2’:3’ cyclic phosphate nucleoside that is hydrolyzed to the corresponding 3’-nucleoside phosphate. It differs from Pancreatic RNase in that it attacks the guanine sites specifically to yield 3′-GMP and oligonucleotides with a 3′-GMP terminal group. |
| Other Notes: | One unit will produce acid soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 15 min at pH 7.5 at 37°C, in a reaction volume of 1.0 mL. Substrate: Yeast RNA. |
| Physical form: | Suspension in 2.8 M (NH4)2SO4 solution |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 300,000-600,000 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.27.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |