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Anti-Rabbit IgG (whole molecule) antibody produced in goat

SIGMA/R1131 - whole antiserum

Synonym: Goat Anti-Rabbit IgG (whole molecule)

MDL Number: MFCD00162781
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-R1131-2ML 2 mL
$140.00
1/EA
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ELISA Anti-Rabbit IgG (whole molecule) antibody produced in Goat (Cat. No. R1131), followed by 1:5,000 Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A5420), was serially diluted to detect Rabbit IgG by indirect ELISA.

 

antibody form whole antiserum
antibody product type secondary antibodies
biological source goat
clone polyclonal
conjugate unconjugated
contains 15 mM sodium azide
Quality Level 200 
shipped in dry ice
storage temp. −20°C
target post-translational modification unmodified
technique(s) indirect ELISA: 1:150,000
  quantitative precipitin assay: 15.0 mg/mL
Application: Anti-Rabbit IgG (whole molecule) antibody produced in goat was used for immunohistochemistry of rat brain sections. It was also used in protein spotting assays.
Biochem/physiol Actions: IgG is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description: Anti-Rabbit IgG is developed in goat using rabbit IgG isolated from normal rabbit serum as the immunogen. IgG is present in large quantities in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γchains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy chains consist of a N-terminal variable domain (VH) and three constant domains (CH1, CH2, CH3). A hinge region exists between the CH1 and CH2 region. The light chains have one N-terminal variable domain (VL) and one constant domain (CL). The heavy and the light chains are linked at VH and CH1 domain to form the Fab arm (Fragment antigen binding). The antigen binds to the V regions of the antibody.
Preparation Note: treated to remove lipoproteins
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352203

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