RNase B Glycoprotein Standard from bovine pancreas
SIGMA/R1153 - Proteomics Grade
Synonym: Ribonuclease B from bovine pancreas; RNase B
CAS Number: 9001-99-4
EC Number: 232-646-6
MDL Number: MFCD00132184
Product Type: Chemical
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| assay | ≥90% (SDS-PAGE) |
| form | lyophilized powder |
| grade | Proteomics Grade |
| InChI | 1S/C9H14N4O3/c10-2-1-8(14 |
| InChI key | CQOVPNPJLQNMDC-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | [nH]1cnc(c1)CC(NC(=O)CCN) |
| storage temp. | −20°C |
| Analysis Note: | RNAse B Glycoprotein Standard has been highly purified to remove contaminating RNase A. |
| Application: | RNase B is a preferred substrate with PNGase F for demonstration of N-linked deglycosylation using SDS-PAGE or MALDI-MS. The activity of PNGase F is routinely assayed using RNase B by monitoring the pronounced mobility shift in 12% gels after deglycosylation. Proteomics Grade RNase B has been used as a source of N-glycans following enzymatic digestions and subsequent purification. It has also been used as a glycoprotein standard. |
| Other Notes: | Bovine pancreatic RNase B is a glycoprotein that contains only N-linked glycans. It is a globular protein composed of a single domain that occurs naturally as a lesser component in mixture along with ribonuclease A (RNase A), which is the non-glycosylated core form. RNase B contains a single glycosylation site at Asn34 at which from five to nine mannose residues are attached to the chitobiose core, i.e. Man5-9GlcNAc2. Due to the heterogeneity in the glycosylation at Asn34, RNase B exists as five glycosylated varients, with a molecular weight of approx. 15 kDa. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |