Ribonuclease Inhibitor
SIGMA/R1158 - recombinant, expressed in E. coli, 20-40 units/μL
Synonym: RNAse inhibitor
MDL Number: MFCD00132187
Product Type: Chemical
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| concentration | 20-40 units/μL |
| form | buffered aqueous solution |
| grade | Molecular Biology |
| mol wt | ~50 kDa |
| Quality Level | 300  |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | Ribonuclease Inhibitor is suitable for use in useful for in vitro inhibition of ribonucleases, including procedures like: • cDNA synthesis from mRNA • reverse transcriptase polymerase chain reaction (RT-PCR) • in vitro transcription and translation • mRNA protection from degradation • formaldehyde cross-linked lncRNA purification • RNA-fluorescence in situ hybridization (FISH) |
| Application: | Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation. |
| Biochem/physiol Actions: | Inhibits RNase by forming a tight, non-covalent 1:1 complex. |
| General description: | Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is derived from E. coli which expresses portions of the human placental ribonuclease inhibitor. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes. The pH range for inhibition is pH 5.5–9 (highestinhibition at pH 7–8). |
| Other Notes: | • Denaturing conditions (i.e., urea or temperatures ≥50 °C) should be avoided as they may cause a release of active ribonuclease from the complex. • This product is for R&D use only, not for drug, household, or other uses. |
| Other Notes: | One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system. |
| Other Notes: | Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol. |
| Physical form: | Solution in 20 mM HEPES-KOH, pH 7.6, 50 mM KCl, 8 mM DTT and 50% glycerol. |
| Symbol |  GHS05 |
| Signal word | Danger |
| Hazard statements | H318 |
| Precautionary statements | P280 - P305 + P351 + P338 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |