Monoclonal Anti-Ryanodine Receptor antibody produced in mouse
SIGMA/R128 - clone C3-33, ascites fluid
Synonym: Anti-CCO; Anti-KDS; Anti-MHS; Anti-MHS1; Anti-PPP1R137; Anti-RYDR; Anti-RYR; Anti-RYR-1; Anti-SKRR
MDL Number: MFCD00282461
Product Type: Chemical
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| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | C3-33, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| isotype | IgG1 |
| mol wt | antigen ~565 kDa (non-mammalian vertebrates, a doublet at 565 kDa represents the α and β isoforms) |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | canine, rabbit, rat, chicken, amphibian, guinea pig, mouse, fish, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (frozen sections): 1 μg/mL using 4% paraformaldehyde-fixed, frozen mouse brain tissue |
| immunoprecipitation (IP): suitable | |
| western blot (chemiluminescent): 1 μg/mL | |
| UniProt accession no. | P21817  |
| Application: | Anti-Ryanodine Receptor has been used in immunofluorescence assay. |
| Biochem/physiol Actions: | In rats PASMCs (pulmonary artery smooth muscle cells), RyR2 (ryanodine receptor isoform 1) participates in RyR-gated Ca2+ entry. It controls the secretion of insulin and glucose homeostasis. After hemorrhagic shock, RyR2 involves in the progress of vascular bi-phasic reactivity to NE (norepinephrine). In human, RyR1 plays an important role in muscle contraction. RYR1 mutation causes CCD (central core disease) and MHS (malignant hyperthermia susceptibility). |
| Biochem/physiol Actions: | The antibody reacts strongly with RyR-2 (expressed predominantly in the heart muscle, but also found in stomach, endothelial cells and diffuse areas of the brain; also known as the β isoform), and weakly with RyR-1 (expressed predominantly in skeletal muscle and areas of the brain; also known as the α isoform). It reacts with ryanodine receptor in cardiac muscle of canine, rat, finch and pigeon; in visceral smooth muscle of toad; and in rat brain. It reacts with skeletal muscle of fish and the β isoform of frog, but only weakly with skeletal muscle in rabbit and the α isoform of frog. In immunohistochemistry, the antibody stains are consistent with the endoplasmic reticulum localization of the receptor in the hippocampus. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | RyR1 (ryanodine receptor isoform 1) is a homotetrameric intracellular calcium channel, that is present in skeletal muscle tissue. It is located on human chromosome 19q13.1. In rats, RyR1 and RyR2 are expressed in PASMCs (pulmonary artery smooth muscle cells). |
| Immunogen: | dog cardiac ryanodine receptors (RyR) |
| Physical form: | Containing PBS and 0.05% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |