Monoclonal Anti-RPA/p34 antibody produced in mouse
SIGMA/R1280 - ~2 mg/mL, clone 9H8, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-REPA2; Anti-RPA2; Anti-RPA32; Anti-Replication protein A2; 32kDa
MDL Number: MFCD03845826
Product Type: Chemical
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with Monoclonal Anti-RPA/p34 (Cat. No. R1280). The antibody was developed with 1:4,000 Goat Anti-Mouse IgG, Peroxidase conjugate (Cat. No. A2304). Lanes 1. 2 μg/mL antibody 2. 1 μg/mL antibody 3. 0.5 μg/mL antibody 4. 0.25 μg/mL antibody 5. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 9H8, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~34 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| western blot: 0.1-0.2 μg/mL using HeLa total cell extract | |
| UniProt accession no. | P15927  |
| Application: | The antibody may be used in: • immunoblotting (~34 kDa) • immunocytochemistry • immunoprecipitation |
| Biochem/physiol Actions: | Replication protein A (RPA) complex is essential for processes like DNA replication, recombination, and DNA repair pathways. The 32-kDa subunit of human RPA is hyper-phosphorylated in its N-terminus upon cellular DNA damage. This phosphorylation causes a change in RPA conformation that down-regulates its activity in DNA replication but does not affect DNA repair processes. Thus the role of RPA phosphorylation in the cellular response to DNA damage is to help regulate DNA metabolism and promote DNA repair.The RPA complex, once bound to single stranded DNA, recruits the ATR (ataxia-telangiectasia mutated and Rad3-related)-ATRIP (ATR-interacting protein) protein kinase complex that is crucial for the cellular response to replication stress and DNA damage and for ATR-mediated Chk1 activation in human cells. Furthermore, the association of this complex to the single stranded DNA causes the phosphorylation of RAD17 protein that is bound to DNA. Yeast strains deficient in RPA are defective in recruiting Ddc2 (yeast homologue of ATRIP) and thus down stream proteins are not phosphorylated. Replication protein A binds with the ssDNA with great affinity and interacts specifically with multiple proteins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-RPA/p34 (mouse IgG1 isotype) is derived from the hybridoma 9H8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified human RPA/p34. Replication protein A Replication protein A (RPA) complex is a single-stranded DNA-binding protein and it is made up of 70-, 32- and 14-kDa subunits. |
| Immunogen: | purified human RPA/p34. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |