Monoclonal Anti-Ryanodine Receptor antibody produced in mouse
SIGMA/R129 - clone 34C, ascites fluid
Synonym: Anti-CCO; Anti-KDS; Anti-MHS; Anti-MHS1; Anti-PPP1R137; Anti-RYDR; Anti-RYR; Anti-RYR-1; Anti-SKRR
MDL Number: MFCD00282461
Product Type: Chemical
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| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 34C, monoclonal |
| conjugate | unconjugated |
| isotype | IgG1 |
| mol wt | antigen 565 kDa (non-mammalian vertebrates, doublet at 565 kDa representing the α and β isoforms) |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, rabbit, canine, mink, bovine, frog, fish, primate, chicken, mouse, sheep |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: suitable |
| immunohistochemistry (frozen sections): 1:1,000 | |
| immunoprecipitation (IP): suitable | |
| western blot: 1:5,000 | |
| UniProt accession no. | P21817  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Application: | Mouse monoclonal clone 34C anti-Ryanodine receptor is used to tag ryanodine receptors for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of ryanodine receptor in studies of calcium flux from the sarcoplasmic reticulum of muscle cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The antibody reacts strongly with RyR-1 (expressed predominantly in skeletal muscle and areas of the brain; also known as the α isoform) and RyR-2 (expressed predominantly in the heart muscle, but also found in stomach, endothelial cells and diffuse areas of the brain; also known as the β isoform). In immunohistochemistry, the antibody stains are consistent with the endoplasmic reticulum localization of the receptor in rat hippocampus. |
| General description: | The ryanodine receptor (RyR) is the channel responsible for the release of Ca2+ from the sarcoplasmic reticulum (SR) in muscle cells and it also plays a role in Ca2+ regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR-1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR-2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus). |
| Immunogen: | partially purified chicken pectoral muscle ryanodine receptors (RyR) |
| Physical form: | Purified IgG1 antibody containing 0.05% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |