RNA Sample Loading Buffer
SIGMA/R1386 - for NA electrophoresis, without ethidium bromide
EC Number: 204-135-8
MDL Number: MFCD00213041
Product Type: Chemical
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| foreign activity | RNase, none detected |
| form | solution |
| grade | Molecular Biology |
| Quality Level | 200  |
| storage temp. | −20°C |
| Application: | RNA sample loading buffer is especially formulated for electrophoresis of RNA on formaldehyde-agarose gels with or without ethidium bromide. Ethidium bromide is not recommended for gel staining prior to Northern blot detection because the presence of ethidium bromide in agarose gels or the loading buffer can cause poor transfer efficiency. |
| Application: | Suitable for use with formaldehyde-agarose gels used in Northern blotting procedures. |
| General description: | RNA loading buffer is used as a tracking dye during RNA electrophoresis. The dye has a slight negative charge and will migrate the same direction as RNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:3 to 1:6 with sample, heat to 65C for ten minutes and chill on ice before loading. |
| Other Notes: | Deionized formamide 62.5% (v/v), formaldehyde 1.14 M, bromphenol blue 200 μg/mL, xylene cyanole 200 μg/mL, MOPS-EDTA-sodium acetate at 1.25× working concentration. |
| Preparation Note: | Deionized formamide 62.5% (v/v), formaldehyde 1.14 M, bromphenol blue 200 μg/mL, xylene cyanole 200 μg/mL, MOPS-EDTA-sodium acetate at 1.25× working concentration. |
| Preparation Note: | Recommended usage: Add 1 volume sample to 2-5 volumes of sample loading buffer and mix well. The sample should be heated to 65 °C for 10 minutes, and then chilled on ice immediately before loading on the gel. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 - H315 |
| Precautionary statements | P301 + P312 + P330 - P302 + P352 |
| Hazard Codes | Xn |
| Risk Statements | 22-38 |
| Safety Statements | 22-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12161700 |