RAF1 (EE) (306-end), active, GST tagged human
SIGMA/R1656 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: CMD1NN; CMD1NN; NS5; NS5; RAF proto-oncogene serine/
Product Type: Chemical
| assay | ≥70% (SDS-PAGE) |
| biological source | fermentation/recombinant |
| form | buffered aqueous glycerol solution |
| manufacturer/tradename | Precision |
| mol wt | ~63 kDa |
| product line | PRECISIO® Kinase |
| Protein ID accession no. | NM 002880   |
| Quality Level | 200 |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| solubility | water: soluble |
| specific activity | 5100-6900 nmol/min·mg |
| storage temp. | −70°C |
| technique(s) | cell based assay: suitable |
| UniProt accession no. | P04049 |
| Biochem/physiol Actions: | Rapidly Accelerated Fibrosarcoma 1(RAF1), a member of MAP kinase kinase kinase (MAP3K) family plays a vital role in cell signaling by directly interacting with GTPases and functions downstream in their signaling pathway. RaF1 is activated by Ras in the presence of growth-promoting signals, leading to the activation of mitogen-activated protein kinase kinase (MEK1/MEK2) and extracellular signal-regulated protein kinases 1 and 2 (ERK1/ERK2) downstream. The activated ERK proteins control gene expression related to cell division, apoptosis, cell differentiation, and cell migration. The gene is mutated or upregulated in prostate and bladder cancer. Also, mutations are associated with Noonan syndrome, which leads to various physical abnormalities like craniofacial dysmorphisms, ocular, and musculoskeletal irregularities, neurocognitive impairment, and cardiac malformations. |
| General description: | Research area: Cell Signaling Rapidly Accelerated Fibrosarcoma (RAF) belongs to receptor tyrosine kinase (RTK) family. RAF exists as three isoforms A, B and C-RAF or RAF-1. RAF1 links Ras family members with mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) signaling cascade. The RAF-1 gene is mapped to the human chromosome at 3p25.2. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 5100-6900 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |