Rhodamine B isothiocyanate
SIGMA/R1755 - BioReagent, mixed isomers, suitable for protein labeling
Synonym: RBITC
CAS Number: 36877-69-7
Empirical Formula (Hill Notation): C29H30ClN3O3S
Molecular Weight: 536.08
EC Number: 253-248-9
MDL Number: MFCD00136007
Linear Formula: C29H30ClN3O3S
Product Type: Chemical
| color | purple to red-brown |
| composition | 9-[2-carboxy-5(or 6)-isothiocyanatophenyl]- |
| description | labeling efficiency with bovine albumin > 70% |
| fluorescence | λex 543 nm; λem 580 nm in methanol |
| form | powder |
| InChI | 1S/C29H29N3O3S.ClH/c1-5-3 |
| InChI key | YVSWPCCVTYEEHG-UHFFFAOYSA |
| mol wt | 536.08 g/mol |
| product line | BioReagent |
| Quality Level | 200 ![]() |
| SMILES string | [Cl-].CCN(CC)c1ccc2c(OC3= |
| solubility | methanol: 10 mg/mL (dark red to very dark red and dark purple to very dark purple) |
| storage temp. | −20°C |
| suitability | suitable for protein labeling |
| Application: | Rhodamine B isothiocyanate is used for the following applications: • Rhodamine isothiocyanate labeling • Rhodamine-labeled synthetic APF • Preparations of protein and fluorescent conjugates • Fluorescent red dye |
| General description: | Rhodamine B isothiocyanate (RhBITC) is an organic fluorescence dye and is widely used as a fluorescent tag in a variety of biological applications. RhBITC is adsorbed onto Ag on silica or polystyrene beads. It exhibits not only a strong surface-enhanced Raman scattering (SERS) signal but also a measurable amount of fluorescence. The RhBITC-modified Ag-deposited silica or polystyrene beads disperse well in ethanol. They are also readily coated in water with polyelectrolytes for their further derivatization with biological molecules of interest that can bind to target molecules. |
| Packaging: | 1 g in glass bottle |
| Packaging: | 100, 500 mg in glass insert |
| Symbol | ![]() GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 + H312 + H332 - H315 - H319 - H334 - H335 |
| Precautionary statements | P280 - P301 + P312 + P330 - P302 + P352 + P312 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 20/21/22-36/37/38-42 |
| Safety Statements | 22-26-45 |
| RIDADR | UN 3335 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352116 |



