Anti-Raf-1/c-Raf antibody, Mouse monoclonal
SIGMA/R2404 - clone RNP1, purified from hybridoma cell culture
Synonym: Anti-CMD1NN; Anti-CRAF; Anti-NS5; Anti-Raf-1; Anti-c-Raf
MDL Number: MFCD01092478
Product Type: Chemical
A. A431 cell extract (Human Epidermoid carcinoma) was separated by SDS-PAGE and probed with (Lane 1) of without (Lane 2) Monoclonal Anti-RAF-1 (Clone RNP1, Cat. No. R2404) followed by Goat Anti-Mouse, Peroxidase conjugate (Cat. No. A2304, Lane 2). B. Localization of RAF-1 protein in the cytoplasm of A431. A431 cells were fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. The cells were incubated with Monoclonal Anti-RAF-1 (Clone RNP1, Cat. No. R2404) and further developed with Rabbit Anti-Mouse IgG, FITC-conjugate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | RNP1, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~75 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunocytochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| western blot: 2.0-4.0 μg/mL using extracts of A431 cell line (human epidermoid carcinoma) | |
| UniProt accession no. | P04049  |
| Application: | Monoclonal Anti-Raf-1/c-Raf antibody has been used in: • capture enzyme linked immunosorbent assay (ELISA) • immunoblotting • immunocytochemistry • double immunofluorescence analysis |
| Biochem/physiol Actions: | Monoclonal anti-Raf-1/c-Raf antibody recognizes human, rat and mouse Raf-1 (~75 kDa). The product may also detect Raf-1 degradation products as additional weaker bands at approx. 50 kDa. |
| Biochem/physiol Actions: | Raf-1 (rapidly accelerated fibrosarcoma) controls cell growth and differentiation. Its oncogenic form can initiate malignant transformation of cells. Raf-1 has a critical role in the Ras/MAP kinase signalling pathway, integrating mitogenic signals in mammalian cells from many growth factors, cytokines and oncogenes, e.g., epidermal growth factor (EGF), platelet derived growth factor (PDGF), insulin, interleukin-2 (IL-2) and IL-3, granulocyte macrophage colony-stimulating factor (GM-CSF) and CSF-1. The regulation of Raf-1 activity is a highly complex and involves multistep process. Inactive Raf-1 is found in the cytosol, and is constitutively associated with the chaperones heat shock protein 90 (Hsp90), p50, and the 14-3-3 protein. Raf-1 activity is regulated by autophosphorylation at threonine residues and by phosphorylation at multiple serine and tyrosine residues by a number of protein kinases, including protein kinase C (PKC), non-receptor tyrosine kinase (Src) and Janus kinase 2 (JAK2). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Raf-1/c-Raf (mouse IgG1 isotype) is derived from the RNP1 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids 334-345 of human Raf-1. |
| General description: | Raf-1 is a serine/threonine kinase that is activated by tyrosine kinases and functions upstream of the MAPK-signaling pathway. Raf-1 inhibits Ras signaling and subsequently modulates cell proliferation and development. c-Raf expression has been linked to the pathogenesis of lung carcinoma. |
| Immunogen: | Synthetic peptide corresponding to amino acids of human Raf-1. |
| Packaging: | 200 μL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |