Ribonuclease inhibitor human
SIGMA/R2520 - Inhibits ribonucleases during transcription and translation experiments
Synonym: RNase Inhibitor; Ribonuclease Inhibitor
MDL Number: MFCD00132187
Product Type: Chemical
Representative image of product packaging.
Representative image of product packaging.
| biological source | human placenta |
| concentration | 30,000-50,000 units/mL |
| form | buffered aqueous solution |
| grade | Molecular Biology |
| mol wt | ~50 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | Suitable for use in • in vitro inhibition of ribonucleases • cDNA synthesis • RT-PCR • in vitro transcription and translation |
| Application: | Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation. |
| Biochem/physiol Actions: | Inhibits RNase by forming a tight, non-covalent 1:1 complex. |
| Biochem/physiol Actions: | Inhibits RNase by forming a tight, non-covalent 1:1 complex. Also inhibits angiogenin, a neovascularization promoter which is homologous with RNase A. |
| General description: | Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is human placenta. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes. |
| Other Notes: | One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system. |
| Other Notes: | Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol. |
| Other Notes: | Solution in 50% glycerol, 20 mM HEPES-KOH, pH 7.6, 50 mM KCl and 8 mM DTT |
| Other Notes: | This inhibitor of RNase activity is isolated from human placenta and supplied as a buffered aqueous glycerol solution. |
| Physical form: | Solution in 50% glycerol, 20 mM HEPES-KOH, pH 7.6, 50 mM KCl and 8 mM DTT |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |