Anti-Rabbit IgG, Native antibody, Mouse monoclonal
SIGMA/R2655 - clone RabT-50, purified from hybridoma cell culture
Product Type: Chemical
Immunoprecipitation
Rabbit Anti-eRF1 (Cat. No. E8156) was used to immunoprecipitate eRF1 from HeLa cell lysate.
(Lane 1) whole antiserum; (Lane 2) 5 mg; (Lane 3) 2.5 mg; (Lane 4) Negative control – without cell lysate; (Lane 5) Negative control – without immunoprecipitating antibody; (Lane 6) Immunoblotting positive control – extract of HeLa probed with Rabbit Anti-eRF1 (Cat. No. E8156).
Panel A. Detection secondary antibody Monoclonal Mouse Anti-Rabbit IgG, clone RabT-50 (Cat. No. R2655) followed by Goat Anti-Mouse IgG, Peroxidase Conjugate (Cat. No. A2304).
Panel B. Detection secondary antibody Goat Anti-Rabbit IgG, Peroxidase Conjugate (Cat. No. A0545)
Rabbit Anti-eRF1 (Cat. No. E8156) was used to immunoprecipitate eRF1 from HeLa cell lysate.
(Lane 1) whole antiserum; (Lane 2) 5 mg; (Lane 3) 2.5 mg; (Lane 4) Negative control – without cell lysate; (Lane 5) Negative control – without immunoprecipitating antibody; (Lane 6) Immunoblotting positive control – extract of HeLa probed with Rabbit Anti-eRF1 (Cat. No. E8156).
Panel A. Detection secondary antibody Monoclonal Mouse Anti-Rabbit IgG, clone RabT-50 (Cat. No. R2655) followed by Goat Anti-Mouse IgG, Peroxidase Conjugate (Cat. No. A2304).
Panel B. Detection secondary antibody Goat Anti-Rabbit IgG, Peroxidase Conjugate (Cat. No. A0545)
| antibody form | purified immunoglobulin |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | RabT-50, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | human, rat, chicken, cat, bovine, monkey, goat, pig, horse |
| species reactivity | rabbit |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: suitable | |
| western blot: 1-2 μg/mL using 10 ng of non-reduced rabbit IgG (Cat. No. I5006) per lane |
| Application: | Anti-Rabbit IgG, Native antibody, Mouse monoclonal has been used in: • immunoblotting • immunocytochemistry • immunohistochemistry • enzyme-linked immunosorbent assay (ELISA) |
| Biochem/physiol Actions: | Anti-Rabbit IgG, Native antibody, Mouse monoclonal reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG. It does not recognize human, monkey, bovine, horse, pig, goat, cat, rat, and chicken IgGs. |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Rabbit IgG, Native antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma RabT-50 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Immunoglobulin G (IgG) is found abundantly in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy and the light chains are linked at VH and CH1 domain to form the Fragment antigen binding (Fab) arm. The antigen binds to the V regions of the antibody. |
| General description: | This antibody reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG. |
| Immunogen: | Enriched fraction of rabbit IgG light chain. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discard if not used within 12 hours. |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |