Anti-Radixin antibody produced in rabbit
SIGMA/R3653 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-DFNB24
MDL Number: MFCD06411912
Product Type: Chemical
Immunoprecipitation Anti-Radixin antibody produced in Rabbit (Cat. No. R3653) was used to immunoprecipitate Radixin from Rat liver extract. Detection antibody: 1:2,000 Anti-Moesin antibody, Mouse monoclonal, Clone: 38/87 (Cat. No. M7060) followed by 1:1,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 10 μg/mL antibody 2. 20 μg/mL antibody 3. Negative Control - without first antibody 4. Negative Control - without extract
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~80 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 10-20 μg using RIPA extract (250 μg) of rat liver |
| indirect immunofluorescence: 10-20 μg/mL using cultured human HeLa cells | |
| western blot: 1-2 μg/mL using whole extract of cultured HeLa cells | |
| western blot: 1-2 μg/mL using whole extract of cultured mouse NIH3T3 cells | |
| UniProt accession no. | P35241  |
| Application: | Anti-Radixin antibody produced in rabbit has been used in • immunoblotting • immunoprecipitation • immunofluorescence staining of cultured cells |
| Biochem/physiol Actions: | In human, knockdown of radixin (RDX) represses the development of glioblastoma cell in vitro and in vivo. It participates in cell motility, invasion and tumor progression. It acts as a membrane-cytoskeletal linker in cell surface structures that are abundant with actin. It is necessary for the arrangement of cortical cytoskeleton. RDX mutation can lead to DFNB24 hearing loss in human. |
| Biochem/physiol Actions: | Radixin is the dominant ezrin-radixin-moesin (ERM) protein in liver bile canaliculi where it is considered to have a critical role in bile conjugated-bilirubin secretion by influencing the cellular localization of the multidrug resistance protein 2 (MRP2). Resting normal human blood cells lack detectable radixin. Radixin is found in classical NK cells. Radixin has closed and open forms corresponding to the inactive and active forms as cross-linkers between actin filaments. It may be phosphorylated and it plays an important role in the activation of the Rho family members by recruiting their positive and negative regulators. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | RDX (radixin) belongs to the ERM (ezrin-radixin-moesin) family. It is present in several normal cells, neoplastic cells, epithelial and lymphoid cells. It is located on chromosome 11. In mouse, RDX is found in the hair cell stereocilia of inner ear. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 400-409 of human radixin with an N-terminal added tripeptide, conjugated to KLH. The corresponding sequence is identical in mouse, rat, and pig and differs by two amino acids in chicken. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |