Reference Dye for Quantitative PCR
SIGMA/R4526 - 100 ×, solution
Synonym: Reference dye for qPCR; ROX
Product Type: Chemical
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| color | red |
| concentration | 100 × |
| form | solution |
| Quality Level | 300  |
| solubility | water: soluble |
| storage temp. | 2-8°C |
| technique(s) | PCR: suitable |
| usage | sufficient for ≥600 reactions |
| Application: | Reference Dye for Quantitative PCR has been used:• in the preparation of mastermix for real time-quantitative polymerase chain reaction (RT-qPCR) • as a component of the reaction mixture for detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR) • for analyzing the degree of cellular DNA contamination by qPCR |
| General description: | Sigma′s Reference Dye for quantitative polymerase chain reaction (qPCR) is a proprietary dye for use with real-time PCR. It is used for the normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR. |
| Other Notes: | Reference Dye for Quantitative PCR is forR&D use only. Not for drug, household, or other uses. |
| Packaging: | 0.3 mL in poly bottle |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106300 |