Ribonuclease A from bovine pancreas
SIGMA/R4642 - (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
Synonym: Pancreatic Ribonuclease; RNAsea; RNase A; Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
CAS Number: 9001-99-4
MDL Number: MFCD00132181
Product Type: Chemical
RNAse catalyzes the endonucleolytic cleavage of RNA to yield nucleoside 3′-phosphates and 3′-phosphooligonucleotides ending in Cp or Up.
| biological source | bovine pancreas |
| concentration | 20-40 mg/mL |
| foreign activity | Endonuclease and exonuclease, none detected |
| NICKase and DNase, none detected | |
| form | (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0) |
| grade | Molecular Biology |
| InChI | 1S/C9H14N4O3/c10-2-1-8(14 |
| InChI key | CQOVPNPJLQNMDC-UHFFFAOYSA |
| mol wt | 13.7 kDa |
| ~13,700 | |
| Quality Level | 200  |
| SMILES string | [nH]1cnc(c1)CC(NC(=O)CCN) |
| storage temp. | −20°C |
| suitability | suitable for |
| Analysis Note: | Protein determined by E. |
| Application: | • RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples. • RNase A is also used in RNA sequence analysis and protection assays. • RNase A has been used as a tool for computer-aided drug design. • RNase A supports the analysis of RNA sequences. • RNase A hydrolyze RNA contained in protein samples. • Purification of DNA is supported by RNase A. |
| Application: | Suitable for: • RNase protection assays • Removal of unspecifically bound RNA • Analysis of RNA sequences • Hydrolysis of RNA contained in protein samples • Plasmid DNA purification |
| Features and Benefits: | Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification. |
| General description: | RNase A is an endoribonuclease that attacks at the 3’OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA. |
| General description: | RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA. |
| Other Notes: | A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 10 ug/mL. Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present. |
| Other Notes: | Activators of RNase A include potassium and sodium salts. RNase A can be inhibited by alkylation of His12 or His119. |
| Other Notes: | RNase A is supplied as a solution of 50% glycerol containing 10 mM Tris-HCl (pH 8.0). |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.27.5 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |