Anti-hnRNP-A2/B1 antibody, Mouse monoclonal
SIGMA/R4653 - clone DP3B3, purified from hybridoma cell culture
Synonym: Anti-Heterogeneous Nuclear Ribonucleoprotein-
MDL Number: MFCD03845440
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-hnRNP-A2/B1 Clone: DP3B3 (Cat. No. R4653). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. G361 4. MDBK 5. MDCK 6. CHO
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | DP3B3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen ~36 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, bovine, hamster, monkey, chicken, mouse, canine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| western blot: 1-2 μg/mL using total cell extract from 293T cell line | |
| UniProt accession no. | P22626  |
| Application: | Monoclonal Anti-hnRNP-A2/B1 antibody produced in mouse been used in : • enzyme linked immunosorbent assay (ELISA) • immunoblotting • immunoprecipitation • immunocytochemistry |
| Biochem/physiol Actions: | In cells, it actively participates in pre-messenger RNA (pre-mRNA) processing, mRNA metabolism and transportation. It is overexpressed in the initial stage of lung cancer and in premalignant lesions. It also participates in neurodegeneration mediated by rCGG (repeats). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-hnRNP-A2/B1 (mouse IgG2a isotype) is derived from the DP3B3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human hnRNP-A2. hnRNP-A2/B1 proteins are located in the nucleus of most tissues cells. However, the hnRNP-A2 protein is found also in the cytoplasm of skin and esophagus cells. In rat brain, hnRNP-A2/B1 proteins are found in neurons in the cerebral cortices, hippocampal formation, olfactory regions, caudate-putamin and the supraoptic nucleus of the hypothalamus. |
| Immunogen: | recombinant human hnRNP-A2. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |