Anti-Rab7 antibody produced in rabbit
SIGMA/R4779 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Ras-Related Protein Rab7
MDL Number: MFCD08705509
Product Type: Chemical
Immunofluorescence
Rat NRK cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin. Fixed cells were stained with 20 μg/mL Anti-Rab7 (Cat. No. R4779) and developed with Goat Anti-Rabbit IgG, FITC conjugate.
Rat NRK cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin. Fixed cells were stained with 20 μg/mL Anti-Rab7 (Cat. No. R4779) and developed with Goat Anti-Rabbit IgG, FITC conjugate.
Western Blotting Whole extract of A-431 Human epithelial carcinoma cells were separated on SDS-PAGE and probed with Anti-Rab7 antibody produced in Rabbit (Cat. No. R4779). The antibody was developed using 1:20,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 2.5 μg/mL antibody 2. 2.5 μg/mL antibody + inhibitor 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~23 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 5-10 μg/mL using rat NRK and mouse NIH-3T3 cells fixed and permeabilized with 3% paraformaldehyde followed by 0.4% saponin |
| western blot: 2.5-5.0 μg/mL using extract of human A431 cells | |
| UniProt accession no. | P51149  |
| Application: | Anti-Rab7 antibody can be used in indirect immunofluorescence and immunoblotting. |
| Application: | Anti-Rab7 antibody produced in rabbit has been used in confocal microscopy. |
| Biochem/physiol Actions: | Anti- Rab7 recognizes human, mouse and rat Rab7. |
| Biochem/physiol Actions: | Rab family of small guanosine triphosphatases (GTPases) are central regulators of membrane trafficking between the different subcellular compartments of the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP-bound inactive and GTP-bound active states. Conversion from one state to the other is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment. Through their effector proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion. Rab7 is also involved in the maturation of late autophagic vacuoles. Among Rab7 effectors are RILP (Rab-interacting lysosome protein) that controls late endosomal and lysosomal transport by mediating the recruitment of dynein/dynactin motors, Rabring7 (Rab7- interacting ring finger protein),(10) and the hVPS34/p150 complex. Anti-Rab7 may be used as a marker for late endosomes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Rab7 is a member of the Rab family of small guanosine triphosphatases (GTPases). Rab proteins contain conserved regions involved in guanine nucleotide binding, and hypervariable COOH-terminal domains with a cysteine motif, implicated in subcellular targeting. Each Rab protein shows a characteristic subcellular distribution. The antibody is affinity-purified using the immunizing peptide immobilized on agarose. |
| General description: | Ras-related protein Rab-7 (Rab7) is a member of the Rab family of small guanosine triphosphatases (GTPases). |
| Immunogen: | Synthetic peptide corresponding to amino acid residues of human Rab7 with C-terminal added cysteine, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |