Anti-hnRNP-L antibody, Mouse monoclonal
SIGMA/R4903 - clone 4D11, purified from hybridoma cell culture
Synonym: Anti-Heterogeneous Nuclear Ribonucleoprotein-L
MDL Number: MFCD03845440
Product Type: Chemical
Western Blotting Whole extract of MDBK cells were separated on SDS-PAGE and probed with Anti-hnRNP-L antibody, Mouse monoclonal, Clone: 4D11 (Cat. No. R4903). The antibody was developed using 1:2,000 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A2179). Lanes 1. 1:2,000 antibody 2. 1:4,000 antibody 3. 1:8,000 antibody 4. 1:16,000 antibody 5. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4D11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~68 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, newt, chicken, human, monkey, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.25-0.5 μg/mL using total cell extract of MDBK cells | |
| UniProt accession no. | P14866  |
| Application: | Anti-hnRNP-L antibody has been used in • immunoblotting • enzyme linked immunosorbent assay (ELISA) • immunoprecipitation • immunocytochemistry • western blotting |
| Biochem/physiol Actions: | Heterogeneous nuclear ribonucleoproteins-L (hnRNP-L) is involved in splicing regulations, internal translation initiation of hepatitis C virus (HCV), stability of the human vascular endothelial growth factor (VEGF) mRNA under hypoxic growth conditions, and intron independent mRNA export. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-hnRNP-L (mouse IgG1 isotype) is derived from the 4D11 hybridoma produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALA/c mice immunized with hnRNP proteins and purified by affinity chromatography on ssDNA-agarose. RNA polymerase II transcripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). hnRNPs consist of protein groups named A to U and many of these protein groups consist of more than one isoform. Group L and M are one of the most abundant groups of proteins. |
| Immunogen: | hnRNP proteins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |