Monoclonal Anti-hnRNP-C1/C2 antibody produced in mouse
SIGMA/R5028 - clone 4F4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-Heterogeneous Nuclear Ribonucleoprotein-
MDL Number: MFCD06798703
Product Type: Chemical
Western Blotting HeLa cells nuclear extract were separated on SDS-PAGE and probed with Monoclonal Anti-hnRNP-C1/C2 antibody produced in Mouse, Clone: 4F4 (Cat. No. R5028). The antibody was developed using 1:1,000 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A2179). Lanes 1. 0.1 μg/mL antibody 2. 0.2 μg/mL antibody 3. 0.4 μg/mL antibody 4. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4F4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 41 kDa |
| antigen 43 kDa | |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | monkey, human, chicken, hamster |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.1-0.2 μg/mL using HeLa cell nuclear extract | |
| UniProt accession no. | P07910  |
| Application: | Monoclonal Anti-hnRNP-C1/C2 antibody produced in mouse has been used in: • antibody microarray • western blotting • enzyme linked immunosorbent assay (ELISA)` • immunoprecipitation • immunocytochemistry |
| Biochem/physiol Actions: | Heterogeneous nuclear ribonucleoproteins C1/C2(HnRNP C1/C2) is involved in protein translation, mRNA biogenesis and transport. The hnRNP-C proteins preferentially bind to uridine-rich RNA sequences. Oligomerization of the protein through leucine rich regions in its C-terminal end is important for RNA binding. Mutation of the hnRNPC gene is implicated in embryonic lethal phenotype. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-hnRNP-C1/C2 (mouse IgG1isotype) is derived from the 4F4 hybridoma produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with RNPs eluted from oligo (dT) cellulose column. Heterogeneous nuclear ribonucleoproteins C1/C2 (HnRNP C1/C2) are synthesized from C2 protein by alternative splicing and have an additional13-amino-acids. It is localized in nucleus and has leucine zipper-like RNA-binding motif. The hnRNP-C proteins have a single ribonucleoprotein (RNP) motif RNA-binding domain (RBD) of 80 to 100 amino acid long. |
| Immunogen: | RNPs eluted from oligo (dT) cellulose column. |
| Physical form: | Solution in phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |