Rat Lung Fibroblasts: RLF, neonatal
SIGMA/R506-05N
Product Type: Product-on-demand
Rat Lung Fibroblasts: RLF
| biological source | rat lung |
| growth mode | Adherent |
| manufacturer/tradename | Cell Applications, Inc |
| morphology | fibroblast |
| packaging | pkg of 500,000 cells |
| Quality Level | 100  |
| relevant disease(s) | asthma; tuberculosis; cancer; cardiovascular diseases |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Analysis Note: | Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi. |
| Application: | Pulmonary function, gene expression, healing & repair process, differentiation, extracellular matrix production, growth factor and cytokine activation, actin reorganization, synthesis of type II collagen, elastin, and proteoglycans, airway remodeling, pharmaceutical development. |
| Biochem/physiol Actions: | Lungs |
| General description: | Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. RLF from Cell Applications, Inc. have been used to demonstrate that exaggerated (compared to adults) NF-κB activation in neonates in response to hyperoxia is mediated by phosphorylation of IκBα on Tyr42 (Wright, 2009). They were also used to elucidate melatonin effects and signaling pathway (Ben Soussia, 2012) and served as a control in studies investigating epithelial-mesenchimal transition as the source of myofibroblasts responsible for idiopathic pulmonary fibrosis (Willis, 2005; Jain, 2007). |
| Other Notes: | Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO |
| Preparation Note: | • 2nd passage, >500,000 cells in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO • Can be cultured at least 10 population doublings |
| Preparation Note: | Please refer to the RLF Culture Protocol . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 41106514 |