Ribonuclease A from bovine pancreas
SIGMA/R5125 - Type III-A, ≥85% RNase A basis (SDS-PAGE), 85-140 Kunitz units/mg protein
Synonym: Pancreatic Ribonuclease; RNAsea; RNase A; Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
CAS Number: 9001-99-4
EC Number: 232-646-6
MDL Number: MFCD00132181
Product Type: Chemical
RNAse catalyzes the endonucleolytic cleavage of RNA to yield nucleoside 3′-phosphates and 3′-phosphooligonucleotides ending in Cp or Up.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: R5125-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: R5125-250MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: R5125-25MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: R5125-500MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: R5125-50MG
| application(s) | diagnostic assay manufacturing |
| assay | ≥85% RNase A basis (SDS-PAGE) |
| biological source | bovine pancreas |
| foreign activity | protease, essentially free |
| form | lyophilized powder |
| impurities | salt, essentially free |
| InChI | 1S/C9H14N4O3/c10-2-1-8(14 |
| InChI key | CQOVPNPJLQNMDC-UHFFFAOYSA |
| mol wt | ~13,700 |
| Quality Level | 300  |
| SMILES string | [nH]1cnc(c1)CC(NC(=O)CCN) |
| specific activity | 85-140 Kunitz units/mg protein |
| storage temp. | −20°C |
| suitability | suitable for molecular biology |
| technique(s) | cell based assay: suitable |
| type | Type III-A |
| Analysis Note: | Protein determined by E. |
| Application: | • RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples. • RNase A is also used in RNA sequence analysis and protection assays. • RNase A has been used as a tool for computer-aided drug design. • RNase A supports the analysis of RNA sequences. • RNase A hydrolyze RNA contained in protein samples. • Purification of DNA is supported by RNase A. |
| Application: | Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess nickel-dependent oxidative cross-linking of a protein. Ribonuclease A from bovine pancreas has also been used in a study to investigate equilibrium constants for nonspecific binding of proteins to DNA. |
| Biochem/physiol Actions: | Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3’ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. |
| Features and Benefits: | Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification. |
| General description: | RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA. |
| Packaging: | 1 g in glass bottle |
| Packaging: | 25, 50, 100, 250, 500 mg in glass bottle |
| Preparation Note: | Salt fractionated and chromatographically purified. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥85% RNase A basis (SDS-PAGE) |
| activity | specific activity: 85-140 Kunitz units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.27.5 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |