Monoclonal Anti-Rab9 antibody produced in mouse
SIGMA/R5404 - ~2 mg/mL, clone Rab9-29, purified immunoglobulin, buffered aqueous solution
MDL Number: MFCD03097193
Product Type: Chemical
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with Monoclonal Anti-Rab9 antibody produced in Mouse, Clone: Rab9-29 (Cat. No. R5404). The antibody was developed using 1:2,500 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A2304). Lanes 1. 4 μg/mL antibody 2. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | Rab9-29, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | ~23 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, hamster, canine, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| western blot: 2 μg/mL using total cell extract of HeLa cells. | |
| UniProt accession no. | P51151  |
| Application: | Monoclonal Anti-Rab9 antibody produced in mouse has been used in: • western blotting • enzyme-linked immunosorbent assay(ELISA) • immunoprecipitation • immunocytochemistry |
| Biochem/physiol Actions: | Monoclonal Anti-Rab9 recognizes human, canine, rat, mouse and hamster Rab9 (approx. 23 kDa) |
| Biochem/physiol Actions: | Ras-related protein Rab-9A (Rab9) guanosine triphosphatase (GTPase) is required for the transport of mannose 6-phosphate receptors (MRPs) from endosomes to the trans-Golgi network (TGN). Rab GTPases are central regulators of membrane trafficking between the different subcellular compartments of the eukaryotic cell. Rab9 GTPase in its GTP bound conformation binds specifically to an effector protein named p40 that stimulates the transport of mannose 6-phosphate receptors (MPRs) from endosomes to the TGN. Rab9 also interacts with the vesicle cargo selection protein tail-interacting protein of 47 kDa (TIP47). This interaction is required for Rab9 stability on late endosomes and may be crucial for vesicular transport in the virus life cycle. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Rab9 (mouse IgG1 isotype) is derived from the hybridoma Rab9-29 produced by the fusion of mouse cells (NS1 cells) and splenocytes from BALB/c mice. Ras-related protein Rab-9A (Rab9) is a member of the Rab family of small guanosine triphosphatases (GTPases). The gene Rab9 is mapped to human chromosome Xp22.2. Rab9 GTPase is localized predominantly to late endosomes. |
| Immunogen: | synthetic peptide corresponding to amino acids 187-200 of human Rab9, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |