Ribonucleic acid, transfer from baker’s yeast (S. cerevisiae)
SIGMA/R5636 - buffered aqueous solution
Synonym: Transfer RNA; tRNA
CAS Number: 9014-25-9
MDL Number: MFCD00132200
Product Type: Chemical
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| concentration | ≥9 mg/mL |
| foreign activity | DNase, Nickase, none detected |
| form | buffered aqueous solution |
| grade | Molecular Biology |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | Suitable for use as a carrier in nucleic acid purification and precipitation. Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used: • in carrier solution as a part of control used for reverse transcriptase – quantitative polymerase reaction (RT-qPCR) assay • as a component of prehybridization(4) and hybridization buffer in single-label in situ hybridization • as a carrier to enhance recovery of RNA from small numbers of cells |
| Biochem/physiol Actions: | Transfer RNAs (tRNAs) play an important role in translation. It is responsible for the addition of amino acids to ribosome for peptide chain formation. The tRNA contains an anticodon region for mRNA base pairing and two attachment regions: for amino acid binding and tRNA synthetase recognition. The ribosomal RNA facilitates the movement of tRNA along the mRNA. |
| General description: | Transfer RNA (tRNA) is isolated from baker′s yeast by phenol-chloroform extraction and ethanol precipitation. tRNAs are approximately 80 nucleotides long RNA molecules with a cloverleaf shaped secondary structure and a tertiary L-shaped structure. |
| Preparation Note: | tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352106 |