Monoclonal Anti-hnRNP-Q antibody produced in mouse
SIGMA/R5653 - clone 18E4, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-Heterogeneous Nuclear Ribonucleoprotein-Q
MDL Number: MFCD03845440
Product Type: Chemical
Immunoblotting
A HeLa cell extract (Human Cervix Epitheloid Carcinoma) was separated by SDS-PAGE, blotted with (Lane 1) or without (Lane 2) Monoclonal Anti-hnRNP-Q (Cat. No. R5653) and developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
A HeLa cell extract (Human Cervix Epitheloid Carcinoma) was separated by SDS-PAGE, blotted with (Lane 1) or without (Lane 2) Monoclonal Anti-hnRNP-Q (Cat. No. R5653) and developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 18E4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 55-70 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, bovine, human, rat, canine, Xenopus, chicken |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1-2 μg/mL using HeLa total cell extract |
| Application: | Monoclonal Anti-hnRNP-Q antibody produced in mouse has been used in immunoblotting and immunofluorescence. It may be used in enzyme linked immunosorbent assay (ELISA), immunoprecipitation and immunohistochemistry. |
| Biochem/physiol Actions: | Heterogeneous nuclear ribonucleoproteins (hnRNPs) act in several biological activities such as transcription, pre- mRNA processing, cytoplasmic mRNA translation and turnover. These proteins interact with the survival of motor neurons protein (SMN) that is mutated in patients with spinal muscular atrophy (SMA). Immunodepletion of hnRNP-Q proteins and their localization in spliceosomes, indicate their important role in splicing. |
| Biochem/physiol Actions: | The hnRNP-Q and hnRNP-R antibodies share 83% homology. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-hnRNP-Q (mouse IgG1) is derived from the 18E4 hybridoma produced by the fusion of murine myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with recombinant human hnRNP-Q. Heterogeneous nuclear ribonucleoproteins-Q (hnRNP-Q) family of proteins consist of three proteins (Q1, 2, and 3) that are derived by alternative splicing from the same gene. |
| Immunogen: | recombinant human hnRNP-Q. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |