RPMI-1640 Medium
SIGMA/R5886 - HEPES Modification, With 25 mM HEPES, without L-glutamine., liquid, sterile-filtered, suitable for cell culture
Synonym: Roswell Park Memorial Institute 1640 medium
MDL Number: MFCD00217820
Product Type: Chemical
| components | L-glutamine: no sodium pyruvate: no HEPES: 25 mM phenol red: yes NaHCO3: yes |
| form | liquid |
| impurities | endotoxin, tested |
| pH | >7.2 |
| Quality Level | 400  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | RPMI-1640 Medium has been used: • to culture human lymphoblastoid cell line cells • prostate cancer cells • monocytes from human blood |
| General description: | RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes. |
| General description: | This RPMI-1640 medium is supplemented with HEPES. HEPES is a Good’s physiological buffer that helps provide a more stable medium pH than bicarbonate buffers. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |