RNase A solution
SIGMA/R6148
Synonym: GenElute™ RNAse A
Product Type: Chemical
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| biological source | bovine pancreas |
| concentration | 20 mg/mL in Tris buffer |
| form | liquid |
| Quality Level | 200  |
| specific activity | 1500 units/mL |
| 3000 units/mL | |
| storage temp. | room temp |
| technique(s) | DNA purification: suitable |
| Application: | RNase A solution has been used: • in apoptosis assay of Hep-2 cells • for the colorimetric quantification of methylated DNA • for DNA and RNA extraction • in DNA laddering analysis • in cell cycle analysis • as a component in phosphate-buffered saline (PBS) to incubate cells for flow cytometry |
| Biochem/physiol Actions: | Ribonuclease A or bovine pancreatic ribonuclease A (RNase A) enzyme is an endoribonuclease that displays a specific ability to cleave single-stranded RNA on the 3′ side of pyrimidine residues. This endonuclease with 124 amino acids plays a key role in gene duplication, cellular metabolism, and cancer therapy. Its overexpression is closely associated with several types of cancer. Hence, RNase A has the potential to be a promising new biomarker for the diagnosis of malignant tumors and is also used as a specific target for new drug discovery. RNase A is commonly used for the isolation of plasmid DNA. |
| General description: | Ribonuclease A (RNase A), a protein composed of three α helices and seven β strands arranged in two "lobes” holds significance as the prototypical domain-swapped protein. It is known for its diverse oligomeric states. The RNase A family derives its name from bovine pancreatic RNase A and is specific to vertebrates. In humans, this family encompasses eight functional members, showcasing its biological relevance. For sample preparation in GenElute™ kits. |
| Legal Information: | GenElute is a trademark of Sigma-Aldrich Co. LLC |
| Preparation Note: | For usage requirements, please refer to the kit procedure. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 1500 units/mL; specific activity: 3000 units/mL |
| Storage Temp. | room temp |
| UNSPSC | 12352204 |