EcoR I from Escherichia coli BS5
SIGMA/R6265 - Restriction Enzyme
CAS Number: 80498-17-5
EC Number: 279-487-9
MDL Number: MFCD00163532
Product Type: Chemical
| concentration | 10,000 units/mL |
| form | buffered aqueous glycerol solution |
| grade | Molecular Biology |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| Application: | EcoRI is a restriction endonuclease that is used in molecular biology applications to cleave DNA at the recognition site 5′-G/AATTC-3′, generating fragments with 5′-cohesive termini. |
| Biochem/physiol Actions: | Recognition sequence: 5′-G/AATTC-3′ Cutting results: a 2-10-fold EcoR I overdigestion of 1 μg λ DNA substrate results in 100% cutting Heat inactivation: 65 °C for 20 minutes. |
| Other Notes: | Comment: Avoid suboptimal reaction conditions such as low salt, high pH (>8.0) and high glycerol (>5%) as they will alter EcoRI specificity and precipitate star activity. |
| Other Notes: | One unit is the enzyme activity that completely cleaves 1 mg of λDNA in 1 hour at 37 °C in a total volume of 50 ml of 1x digestion buffer SH for restriction enzymes. 1 mg pBR322 DNA is digested completely by 2 units of EcoR I. |
| Other Notes: | Supplied with 10x Restriction Enzyme Buffer SH (B3657). |
| Physical form: | Solution in 10 mM Tris-HCl, pH 7.2 , 1 mM EDTA, 200 mM NaCl , 0.5 mM dithioerythritol, 50% glycerol (v/v), 0.2% Triton X- 100 (v/v), 4°C |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.21.4 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |