Monoclonal Anti-phospho-Retinoblastoma (pSer⁷⁹⁵) antibody produced in mouse

SIGMA/R6878 - ~2 mg/mL, clone RB-10, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-phospho-Rb (pSer⁷⁹⁵)

MDL Number: MFCD03457570
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-R6878-.1ML	0.1 mL	$748.00 1/EA	Add To Favorites

Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-phospho-Retinoblastoma (pSer⁷⁹⁵) Clone: RB-10 (Cat. No. R6878). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. G361 2. COS7 3. ROS

Immunoprecipitation Monoclonal Anti-phospho-Retinoblastoma (pSer⁷⁹⁵) Clone: RB-10 (Cat. No. R6878) was used to immunoprecipitate phospho-Retinoblastoma from G361 whole cell extract. Detection antibody: Monoclonal Anti-phospho-Retinoblastoma (pSer⁷⁹⁵) Clone: RB-10 (Cat. No. R6878). Lanes 1. 5 μg antibody 2. 2.5 μg antibody 3. Negative control: 5 μg non-relevant antibody 4. Positive control: G361 whole cell extract

Properties
Descriptions
Basic Data

antibody form	purified immunoglobulin
antibody product type	primary antibodies
biological source	mouse
clone	RB-10, monoclonal
concentration	~2 mg/mL
conjugate	unconjugated
form	buffered aqueous solution
isotype	IgG1
mol wt	antigen 110 kDa
Quality Level	200
shipped in	dry ice
species reactivity	mouse, rat, human, bovine, hamster
storage temp.	−20°C
target post-translational modification	phosphorylation (pSer795)
technique(s)	immunohistochemistry: suitable using 3% paraformaldehyde fixation, 0.15% Triton X-100 permeabilization
	indirect ELISA: suitable
	microarray: suitable
	western blot: 2-4 μg/mL using a whole cell extract of human melanoma cell line G-361
UniProt accession no.	P06400

Application:	Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper)
Application:	Monoclonal Anti-phospho-Retinoblastoma (pSer⁷⁹⁵) antibody produced in mouse has been used in: • immunoblotting • immunocytochemistry • immunofluorescence • fluorescence-activated cell sorting (FACS) assays • enzyme linked immunosorbent assay (ELISA)
Biochem/physiol Actions:	Retinoblastoma (Rb) exerts its function by repressing the transcription of cellular genes required for DNA replication and cell division. It can be phosphorylated at multiplicity of sites in vitro by cell division cycle protein 2 (Cdc2), cyclin-dependent kinase (Cdk2) and Cdk4/Cdk6 kinase complexes. Phosphorylation of pS⁷⁹⁵ is required to inactivate Rb imposed growth suppression.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description:	Monoclonal Anti-phospho-Retinoblastoma (Rb) (pS795) (mouse IgG1 isotype) is derived from the RB-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic phospho peptide corresponding to the C-terminal amino acids of human retinoblastoma protein, conjugated to KLH. Retinoblastoma proteins p107, p110 (also designated Rb, or pRb) and p130, referred to collectively as pocket proteins, constitute a nuclear protein family that share a common structural unit (the pocket) dedicated to binding certain transcription factors and thereby regulating cellular proliferation. Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with conserved LXCXE motif.
Immunogen:	synthetic phosphopeptide corresponding to the C-terminal amino acids 791-804 (pSer⁷⁹⁵) of human retinoblastoma protein conjugated to KLH.
Physical form:	Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage Temp.	−20°C
UNSPSC	12352203

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