RPMI-1640 Medium
SIGMA/R7509 - Modified, with sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
Synonym: Roswell Park Memorial Institute 1640 medium
MDL Number: MFCD00217820
Product Type: Chemical
RPMI-1640 Medium This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
| components | phenol red: no L-glutamine: no HEPES: no sodium pyruvate: no NaHCO3: yes |
| form | liquid |
| impurities | endotoxin, tested |
| Quality Level | 400  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | RPMI-1640 Medium has been used:• to culture Mantle cell lymphoma cells • to culture mouse podocytes • in the extraction of whole bone marrow from rat Femur and tibia bones |
| General description: | RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes. |
| General description: | RPMI-1640 Medium is suitable for culturing human normal and neoplastic leukocytes. |
| Other Notes: | Phenol red has been shown to interfere with the growth of some cells at clonal densities. Use this medium when working with stem cells or when growing cells at low densities. Also recommended for in vitro diagnostics use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |