Random Nonamers
SIGMA/R7647 - for use as primers in cDNA synthesis, 50 μM in H2O
Synonym: Oligodeoxynucleotides
Product Type: Chemical
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| concentration | 50 μM in H2O |
| form | liquid |
| grade | Molecular Biology |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| usage | 0.1 mL sufficient for 100 RT-PCR reactions |
| Application: | Random Nonamers are suitable for: • First-strand cDNA synthesis • Preparing cDNA libraries (with oligo (dT) primers • Prepare labeled DNA probes for hybridizations |
| Features and Benefits: | • When preparing cDNA libraries or dealing with situations where specific primers are not suitable, the random nonamers can be combined with anchored oligo (dT)23 primers (Product No. O4387). • In cases of incomplete or missing sequence information, or when specific primers are not effective, the random nonamers offer a solution for cDNA library preparation. • The random nonamers′ priming capability at higher temperatures (up to 65°C) is reduced, ensuring that they do not interfere with polymerase chain reaction(PCR) following transcription. • Randomnonamers can also be used to prepare labeled DNA probes for hybridizationstudiesRando |
| General description: | Random Nonamers are random sequences of nine deoxyribonucleotides (9-mers) used to prime mRNA, with or without a poly(A) tail. Random nonamers may be used as universal, non-specific primers. |
| Other Notes: | For laboratory use only. Not for drug, household, or other uses. |
| Other Notes: | Random Nonamers are provided as a 50 uM solution in water. |
| Packaging: | 100 μL in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41105500 |