Red Blood Cell Lysing Buffer Hybri-Max™
SIGMA/R7757 - liquid, sterile-filtered, suitable for hybridoma
Synonym: RBC Lysing Buffer
Product Type: Chemical
| form | liquid |
| impurities | endotoxin, tested |
| Quality Level | 400  |
| sterility | sterile-filtered |
| technique(s) | cell culture | hybridoma: suitable |
| Application: | Recommended for use by adding 1 mL of buffer to a cell pellet (cell pellet = 1 spleen or 100-200 million cells). Gently mix for 1 minute. Dilute the buffer with 15-20 mL of medium or salt solution. Centrifuge at 250-500 × g for 7 minutes and decant the supernatant. Cells may be diluted and prepared for counting or fusion. If lysis is incomplete, steps 1-4 may be repeated. |
| Biochem/physiol Actions: | Red Blood Cell Lysing Buffer has been developed for use in hybridoma protocols to remove red blood cells from mouse splenocyte suspensions before fusion. It is also useful in systems where it may be desirable to remove red blood cells from cell suspensions, such as whole blood. |
| Legal Information: | Hybri-Max is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Note: This product is intended for the removal of red blood cells from mice. This product may not be appropriate for the lysis of red blood cells of other animals. The suitability of the product in any application other than mouse splenocytes must be determined by the researcher. |
| Preparation Note: | Contains 8.3 g/L ammonium chloride in 0.01 M Tris-HCl buffer. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12352207 |