Ribonucleic acid, transfer from wheat germ
SIGMA/R7876 - Type V, 15-19 units/mg solid
Synonym: Transfer RNA; tRNA
CAS Number: 9014-25-9
MDL Number: MFCD00132200
Product Type: Chemical
Ribonucleic acid, transfer from wheat germ illustration
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: R7876-10KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: R7876-2.5KU
| form | lyophilized powder |
| grade | Molecular Biology |
| Quality Level | 300  |
| solubility | H2O: ~2 mg/mL, clear to hazy |
| specific activity | 15-19 units/mg solid |
| storage condition | desiccated |
| storage temp. | 2-8°C |
| type | Type V |
| Application: | Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly. |
| Application: | Suitable for use as a carrier in nucleic acid purification and precipitation. |
| General description: | Wheat germ tRNA contains many different specific tRNA molecules. |
| Other Notes: | One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path). |
| Packaging: | 2500, 10000 units in glass bottle |
| Packaging: | 500 units in poly bottle |
| Preparation Note: | tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 15-19 units/mg solid |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352106 |