Ribonuclease B from bovine pancreas
SIGMA/R7884 - BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE)
Synonym: RNase B
CAS Number: 9001-99-4
EC Number: 232-646-6
MDL Number: MFCD00132184
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: R7884-100MG
| application(s) | cell analysis |
| assay | ≥80% (SDS-PAGE) |
| biological source | bovine pancreas |
| concentration | ≥60% |
| foreign activity | protease ≤0.001 units/mg solid |
| form | powder |
| InChI | 1S/C9H14N4O3/c10-2-1-8(14 |
| InChI key | CQOVPNPJLQNMDC-UHFFFAOYSA |
| product line | BioReagent |
| Quality Level | 200  |
| SMILES string | [nH]1cncc1CC(NC(=O)CCN)C( |
| specific activity | ≥50 Kunitz units/mg protein |
| storage temp. | −20°C |
| suitability | suitable for molecular biology |
| technique(s) | cell based assay: suitable |
| Application: | Ribonuclease B from bovine pancreas is used in the digestion of RNA during cell cycle platform analysis. |
| Biochem/physiol Actions: | Native RNase BS generated by subtilisin digestion of native RNase B comprising of amino acid residues 21-124 of RNase B, is sensitive to PNGase F digestion. Intramolecular N-glycans of bovine pancreatic RNase B function like chaperone. RNase B is found to be much faster than RNase A, while RNase A is liable to aggregate during regeneration. The stimulatory effect of Asn-oligosaccharide (which corresponds to the most predominant sugar chain of RNase B) reveals that the N-glycans of RNase B facilitates the transformation of bulky intermediates into folded, compact species. |
| Packaging: | 100 mg in poly bottle |
| Packaging: | 500 μg in poly bottle |
| Packaging: | Package size based on protein content |
| Preparation Note: | Purified by affinity chromatography |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥80% (SDS-PAGE) |
| activity | specific activity: ≥50 Kunitz units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.27.5 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |