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D-Ribulose 1,5-Diphosphate Carboxylase from spinach

SIGMA/R8000 - partially purified powder, 0.01-0.1 unit/mg solid

Synonym: 3-Phospho-D-glycerate carboxy-lyase(dimerizing); Rubisco

CAS Number: 9027-23-0
MDL Number: MFCD00130908
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-R8000-1UN 1 unit
 $218.00
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45-R8000-5UN 5 units
 $827.00
1/EA		 Add To Favorites
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: R8000-5UN

 

biological source spinach
form partially purified powder
mol wt 557 kDa
Quality Level 200 
specific activity 0.01-0.1 unit/mg solid
storage temp. −20°C
Application: D-Ribulose 1,5-Diphosphate Carboxylase from spinach has been used:
• as a test protein in pepsin digestion studies
• as an innocuous or non-hazardous protein sample to test its effect on human intestinal epithelial cell lines
• in isothermal titration calorimetry (ITC), and radiolabeled binding assays with abscisic acid
Biochem/physiol Actions: D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) depends on Rubisco activase and chaperones for activation. It participates in plant photorespiration events by catalyzing the carboxylation and oxygenation of ribulose-1,5-bisphosphate. Abscisic acid inhibits the carboxylation activity of Rubisco.
General description: D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) amounts to 50% of the total spinach leaves associated soluble protein.
General description: Exists as a 557 kDa hexadecamer composed of eight heavy chains each with a molecular weight of approximately 56 kDa and eight light chains of molecular weight 14 kDa. Each molecule contains one magnesium ion.
pH optimum: ~7.9.
KM for CO2: ~0.45 mM.
Ribulose diphosphate becomes inhibitory at concentrations exceeding 0.7 mM. Orthophosphate and ammonium sulfate are competitive inhibitors. 3-Phosphoglycerate is a noncompetitive inhibitor.
Other Notes: One unit will convert 1.0 μmole of D-RuDP and CO2 to 2.0 μmoles of D-3-phosphoglycerate per min at pH 7.8 at 25°C.
Packaging: 1 unit in poly bottle
Packaging: 5 units in poly bottle
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
activity specific activity: 0.01-0.1 unit/mg solid
Storage Temp. −20°C
Enzyme Commission (EC) Number 4.1.1.39   ( BRENDA  | IUBMB  )
UNSPSC 12352204

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