Rhodamine 123
SIGMA/R8004 - mitochondrial specific fluorescent dye
Synonym: 2-
CAS Number: 62669-70-9
Empirical Formula (Hill Notation): C21H16N2O3 · HCl
Molecular Weight: 380.82
EC Number: 263-687-8
MDL Number: MFCD00012664
Linear Formula: C21H17ClN2O3
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: R8004-25MG.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: R8004-5MG.
| application(s) | diagnostic assay manufacturing hematology histology |
| form | powder |
| InChI | 1S/C21H16N2O3.ClH/c1-25-2 |
| InChI key | MYFATKRONKHHQL-UHFFFAOYSA |
| Quality Level | 100  |
| SMILES string | Cl[H].COC(=O)c1ccccc1C2=C |
| solubility | ethanol: 1 mg/mL |
| storage temp. | room temp |
| Application: | Rhodamine 123 is useful as a laser dye and to have selective cell growth effects. It has also been used to measure mitochondrial membrane potential. |
| Biochem/physiol Actions: | Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin |
| Biochem/physiol Actions: | Rhodamine 123 is a fluorescent dye most commonly used in flow cytometry as functional reporter for P-glycoprotein (Pgp). It is part of the rhodamine family of flurone dyes. The dye is mostly used to assess mitochondrial bioenergetics in living cells. Functional assays for multi-drug resistance (MDR) proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin |
| Packaging: | 5, 25, 100 mg in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |