Ribonucleic acid, transfer from baker’s yeast (S. cerevisiae)
SIGMA/R8508 - buffered aqueous solution
Synonym: Transfer RNA; tRNA
CAS Number: 9014-25-9
MDL Number: MFCD00132200
Product Type: Chemical
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| concentration | 9-11 mg/mL |
| foreign activity | DNase, Nickase, none detected |
| form | buffered aqueous solution |
| grade | Molecular Biology |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) is suitable for use as a carrier in nucleic acid purification and precipitation. |
| Application: | Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used to precipitate mRNA of denuded oocytes and for reverse transcription. It has also been used to seal magnetic beads in RNA pull-down assay of nasopharyngeal carcinoma (NPC) cells. |
| Biochem/physiol Actions: | Transfer RNA (tRNA) acts as an ‘adaptor′ molecule that translates the three-nucleotide codon sequence in the mRNA to the corresponding amino acid of that codon. It determines the genetic code by linking amino acids and nucleic acids. tRNA is involved in viral replication, bacterial cell wall biosynthesis, cell stress response, and animal behavior control. Aberrations in tRNA molecules are associated with several human diseases. |
| General description: | Transfer RNA (tRNA) is isolated from baker’s yeast. |
| General description: | Transfer RNA (tRNA) is a short nucleotide RNA chain, which has an L-shaped structure. |
| Preparation Note: | tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352106 |