Anti-Peroxiredoxin 2 (C-terminal) antibody produced in rabbit
SIGMA/R8656 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-NKEFB; Anti-Natural killer-enhancing factor B; Anti-PRDX2; Anti-TDPX1; Anti-Thiol-specific antioxidant 1; Anti-Thioredon peroxidase 1; Anti-Torin
Product Type: Chemical
Immunoblotting Whole extract of HeLa cells was separated on SDS-PAGE and probed with Rabbit Anti-Peroxiredoxin 2 (C-Terminal) (Cat. No. R8656). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 1 μg/mL antibody + Peroxiredoxin 2 immunizing peptide (human, 184-198)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~22 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2.0-5.0 μg using lysate of mouse 3T3 cells |
| western blot: 0.5-1.0 using whole extract of human HeLa cells | |
| western blot: 1-2 μg/mL using whole extract of rat NRK cells | |
| UniProt accession no. | P32119  |
| Application: | Anti-Peroxiredoxin 2 (C-terminal) antibody produced in rabbit has been used in immunoblottingand immunoprecipitation. |
| Biochem/physiol Actions: | Peroxiredoxin 2 has dual roles as a peroxireductase in moderately oxidative conditions and as a molecular chaperone that binds and protects denatured proteins in hyper-oxidative conditions. Peroxiredoxin 2 is involved in platelet-derived growth factor (PDGF) and tumor necrosis factor (TNF) signaling regulation and is elevated in several human cancers and neurodegenerative disorders. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Peroxiredoxin 2 is a cytosolic member of the peroxiredoxin family of antioxidant enzymes. All peroxiredoxin enzymes exist as homodimers, they contain a conserved Cys residue corresponding to Cys51 in mammalian peroxiredoxin 1 and 2 and are distributed differentially within cells. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 184-198 of human peroxiredoxin 2 conjugated to KLH. The corresponding sequence is identical in rat and mouse peroxiredoxin 2. |
| Physical form: | Solution in 0.01 M phophate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |