Anti-Rab7 antibody, Mouse monoclonal
SIGMA/R8779 - ~2 mg/mL, clone Rab7-117, purified from hybridoma cell culture
Synonym: Rab7 Antibody; Rab7 Antibody - Monoclonal Anti-Rab7 antibody produced in mouse; Anti-Ras-associated protein
MDL Number: MFCD08705596
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% saponin. Fixed cells were stained with 10 μg/mL Monoclonal Anti-Rab7 Clone: Rab7-117 (Cat. No. R8779). The antibody was developed using Goat Anti-Mouse IgG, Atto-488 conjugate.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with1 μg/mL Monoclonal Anti-Rab7 Clone: Rab7-117 (Cat. No. R8779). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa; 2. HepG2; 3. NRK; 4. 3T3; 5. MDCK
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | Rab7-117, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | ~24 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, human, monkey, mouse, chicken, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.5-1 μg/mL using HeLa total cell extract | |
| UniProt accession no. | P51149  |
| Application: | Monoclonal Anti-Rab7 antibody produced in mouse may be used in ELISA, immunoblotting (~24 kDa), immunoprecipitation, immunohistochemistry, immunofluorescence and immunocytochemistry. |
| Biochem/physiol Actions: | Monoclonal Anti-Rab7 recognizes human, monkey, bovine, rat, mouse, and chicken Rab7. |
| Biochem/physiol Actions: | Rab GTPases are central regulators of membrane trafficking between different subcellular compartments in eukaryotic cells. The regulatory capacity of Rab GTPases depends on their ability to cycle between the GDP-bound inactive and GTP-bound active states. Activation of Rab proteins is coupled to their association with intracellular membranes, allowing them to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment. Through their effector proteins, Rab GTPases regulate vesicle formation, actin and tubulin-dependent vesicle movement, and membrane fusion. Rab7 regulates vesicle traffic from early to late endosomes, and from late endosomes to lysosomes. Rab7 is also involved in the maturation of late autophagic vacuoles. Among Rab7 effectors are Rab-interacting lysosome protein (RILP) that controls late endosomal and lysosomal transport by mediating the recruitment of dynein/dynactin motors, Rab-7-interacting ring finger protein (Rabring7) and the hVPS34/p150 complex. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Rab7 is a member of the Rab family of small guanosine triphosphatases (GTPases). This family belongs to the rat sarcoma (Ras) superfamily of small GTPases. Rab proteins contain conserved regions involved in guanine nucleotide binding and hypervariable COOH-terminal domains with a cysteine motif, implicated in subcellular targeting. Post-translational modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins. The gene Rab7a in human chromosome is mapped to 3q21.3. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |