Anti-Rat IgG (whole molecule) antibody produced in rabbit
SIGMA/R9255 - IgG fraction of antiserum, buffered aqueous solution
MDL Number: MFCD00162808
Product Type: Chemical
ELISA Anti-Rat IgG (whole molecule) antibody produced in Rabbit (Cat. No. R9255), followed by 1:5,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545), was serially diluted to detect Rat IgG by indirect ELISA.
| antibody form | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 7-14 mg/mL (by absorbance at 280 nm) |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:100,000 |
| quantitative precipitin assay: 2.5-5 mg/mL |
| Application: | Anti-Rabbit IgG (whole molecule) antibody produced in rabbit was used in to precipitate and quantify the titer of human growth hormone and anti-human growth hormone complexes at a dilution of 1:20. |
| Application: | Anti-Rat IgG (whole molecule) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |