Angiotensin II EIA Kit
SIGMA/RAB0010 - for serum, plasma, culture supernatant and cell lysates
Synonym: Angiotensinogen; Serpin A8
Product Type: Chemical
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| assay range | inter-assay cv: <15% intra-assay cv: <10% sensitivity: 2.62 pg/mL standard curve range: 0.1-1000 ng/mL |
| detection method | colorimetric |
| input | sample type cell lysate sample type culture supernatant(s) sample type serum sample type plasma |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | ELISA: suitable |
| enzyme immunoassay: suitable |
| Application: | Angiotensin II EIA Kit has been used for quantitative estimation of serum angiotensin-II. |
| Application: | For research use only. Not for use in diagnostic procedures. Angiotensin II EIA Kit has been used for analyzing circulating and serum levels of angiotensin II (Ang II) in mice and rats respectively. |
| Application: | Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | Study on in vitro model shows that mechanical stretch induced Ang II release plays a vital role in mediating stretch-induced hypertrophic response. Ang II is implicated in maintaining blood pressure and has an important role in hypertension and in the pathophysiology of cardiovascular disorders. Ang II also has an essential role in growth induction, cell migration and mitosis of vascular smooth muscle cells. It enhances the production of collagen type I and III in fibroblasts, that results in thickening of the vascular wall and myocardium, and fibrosis. Variation in Ang II gene increases the risk of myocardial infarction, hypertension and left ventricular hypertrophy. Ang II expression is associated with heart failure, cardiac remodeling, diabetes, and the proliferative and inflammatory responses to arterial injury. The pharmacological agents such as angiotensin II-converting enzyme inhibitors or angiotensin II receptor antagonists can be used for treating Ang II-induced hypertension and congestive heart failure. |
| General description: | The Angiotensin II Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Angiotensin II peptide based on the principle of Competitive Enzyme Immunoassay. EIA-ANGII-1 can theoretically detect all active angiotensins, including ANGI, ANGII, ANGIII and ANGIV. However, it does not detect inactive angiotensinogen. |
| Immunogen: | Angiotensin II, synthetic peptide |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| Precautionary statements | P234 - P390 |
| RIDADR | UN 3264 8 / PGIII |
| WGK Germany | WGK 3 |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |
| Components | HRP-Streptavidin |