Human Angiopoietin-2 ELISA Kit
SIGMA/RAB0016 - for serum, plasma, cell culture supernatant and urine
Synonym: Angiopoietin-2
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human ANGIOPOIETIN-2 ELISA Kit, Cat. No. RAB0016. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human ANGIOPOIETIN-2 ELISA Kit, Cat. No. RAB0016, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 10 pg/mL standard curve range: 4.12-3000 pg/mL |
| detection method | colorimetric |
| input | sample type urine sample type serum sample type cell culture supernatant(s) sample type plasma |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | Angiopoietin-2 (ANGPT2) belongs to the family of angiogenic factors. ANGPT2 is a competitive antagonist of ANGPT1 and reduces vascular stabilization. ANGPT2 is a potent marker for liver cancer. ANGPT2 binds with tyrosine kinase with immunoglobulin-like and endothelial growth factor (EGF)-like domains 2 (Tie-2) and causes neoangiogenesis in liver cirrhosis. Upregulation of ANGPT2 might have an adverse effect on kidney physiology and function. Overexpression of ANGPT2 leads to endothelial damage and dysfunction resulting in diabetic nephropathy in diabetic patients. ANGPT2 is a potent marker for detecting lung cancer progression. Upregulation of ANGPT2 promotes signal transduction for tumor progression in epithelial ovarian cancer. |
| General description: | The Human Angiopoietin-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Angiopoietin-2 in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human Angiopoietin-2 |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Assay/Sample Diluent Buffer E (Item E2); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |