Human Amphiregulin ELISA Kit
SIGMA/RAB0019 - for serum, plasma, cell culture supernatant and urine
Synonym: AR
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human Amphiregulin ELISA Kit, Cat. No. RAB0019. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human Amphiregulin ELISA Kit, Cat. No. RAB0019, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 10 pg/mL standard curve range: 16.46-4000 pg/mL |
| detection method | colorimetric |
| input | sample type plasma sample type cell culture supernatant(s) sample type urine sample type serum |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA)Human Amphiregulin ELISA Kit has been used to estimate the plasma concentration of amphiregulin by ELISA. |
| Biochem/physiol Actions: | Areg (amphiregulin) stimulates growth-signaling pathways via EGF (epidermal growth factor) receptors. Areg participates in the organogenesis of mammary gland. It is regarded as a pro-oncogenic factor and contributes to tumor progression. Upregulation of the AREG gene is observed in cancer and resists chemotherapy. AREG reduces E-cadherin expression in order to promote invasion and progression of ovarian cancer. |
| General description: | The Human Amphiregulin (AR) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human AR in serum, plasma, cell culture supernatants and urine. The AREG (amphiregulin) gene codes for a shedding ectodomain, derived from a transmembrane precursor of 252 amino acids. The encoded protein is released into blood or cellular vicinity through autocrine and paracrine. Areg is predominantly expressed in epithelial, mesenchymal cells and also by active immune cells. The AREG gene is mapped to human chromosome 4q13.3. |
| Immunogen: | Recombinant Human Amphiregulin |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |