Human BMP2 ELISA Kit
SIGMA/RAB0028 - for serum, plasma, cell culture supernatant and urine
Synonym: BMP-2; Bone morphogenetic protein 2
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human BMP2 ELISA Kit, Cat. No. RAB0028. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human BMP2 ELISA Kit, Cat. No. RAB0028, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 45 pg/mL standard curve range: 46.88-3000 pg/mL |
| detection method | colorimetric |
| input | sample type serum sample type cell culture supernatant(s) sample type urine sample type plasma |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Human BMP2 (bone morphogenetic protein 2) ELISA kit has been used for measuring concentrations of BMP in conditioned mouse organoid media. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | BMP2 (bone morphogenetic protein 2) plays a vital role in the activation of the autonomic lineage-specific basic–helix-loop-helix protein MASH1 (mammalian achaete scute homolog-1) and in the autonomic neurogenesis in neural crest stem cells in vitro. During bone repair, BMP-2 controls the expression of Runt-related transcription factor 2 (Runx2) and Osterix (Osx), thereby enhancing migration, proliferation and osteogenic differentiation of mesenchymal stem cells. In addition to its osteogenic activity, BMP-2 also plays an important role in cardiac morphogenesis. Recombinant human bone morphogenetic protein-2 (rhBMP-2) plays an essential role in pathogenesis of oral squamous cell carcinomas. Mutation in BMP-2 gene is associated with osteoporosis. |
| General description: | BMP2 (bone morphogenetic protein 2) is encoded by the gene mapped to human chromosome 20p12.3. The encoded protein belongs to transforming growth factor-β (TGFβ) superfamily. BMP-2 is expressed in heart, lung, and dorsal aorta. |
| General description: | The Human BMP-2 ( Bone morphogenetic proteins-2) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme- linked immunosorbent assay for the quantitative measurement of human BMP-2 in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human BMP2 |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |