Human Carbonic Anhydrase IX ELISA Kit
SIGMA/RAB0036 - for serum, plasma, cell culture supernatant and urine
Synonym: CA9
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human Carbonic Anhydrase IX ELISA Kit, Cat. No. RAB0036. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human Carbonic Anhydrase IX ELISA Kit, Cat. No. RAB0036, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 0.2 pg/mL standard curve range: 0.205-50 pg/mL |
| detection method | colorimetric |
| input | sample type plasma sample type serum sample type cell culture supernatant(s) sample type urine |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | Carbonic anhydrase IX (CA9) aids in reversible hydration of carbon dioxide to carbonic acid and bicarbonate. CA9 functions as a promising biomarker for diagnosing clear cell renal cell carcinoma (ccRCC), tumor hypoxia and prostate cancer (PC). In addition to pH control, CA9 also plays a vital role in cell proliferation, adhesion and tumor invasion. |
| General description: | Carbonic anhydrase IX (CA9) belongs to the zinc-containing metalloenzymes family. The protein is encoded by the gene mapped to human chromosome 9p. The encoded protein is localized on cell membrane and is characterized with an N-terminal proteoglycan-like domain (PG), a CA catalytic domain (CA), a transmembrane helical segment and a short intra-cytoplasmic tail. CA9 is expressed at very low levels in normal tissues (mostly in gastrointestinal tract), but it is expressed at high level in various cancer tissues, such as renal, lung, cervical, ovarian, esophageal and breast cancer. |
| General description: | The Human CA-IX (Carbonic Anhydrase IX) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CA-IX in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human CA9 |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA 1X Assay/Sample Diluent Buffer C (Item L); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |