Human MDC / CCL22 ELISA Kit
SIGMA/RAB0064 - for serum, plasma, cell culture supernatant and urine
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human MDC / CCL22 ELISA Kit, Cat. No. RAB0064. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human MDC / CCL22 ELISA Kit, Cat. No. RAB0064, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 1.5 pg/mL standard curve range: 1.65-400 pg/mL |
| detection method | colorimetric |
| input | sample type cell culture supernatant(s) sample type urine sample type plasma sample type serum |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | Macrophage-derived chemokine (MDC) signals through the C-C chemokine receptor type 4 (CCR4). It chemo-attracts monocytes, dendritic cells and natural killer (NK) cells and aids in their function. The protein exerts human immunodeficiency virus (HIV) suppressive activity. The 67 amino acid form of MDC displays reduced chemoattractant activity but retains HIV suppressive activity. It is expressed in various cancers and is involved in recruitment of regulatory T cells (Treg) within a tumor. |
| General description: | Human MDC / CCL22 enzyme-linked immunosorbent assay (ELISA) kit is an in vitro assay for the quantitative measurement of macrophage-derived chemokine (MDC) protein in biological samples, such as serum, plasma, cell culture supernatants, urine, and/or cell and tissue lysates. |
| Immunogen: | Recombinant Human MDC |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |