Mouse CTACK / CCL27 ELISA Kit
SIGMA/RAB0071 - for serum, plasma and cell culture supernatant
Product Type: Chemical
Standard Curve : Typical Standard Curve for Mouse CTACK / CCL27 ELISA Kit, Cat. No. RAB0071. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Mouse CTACK / CCL27 ELISA Kit, Cat. No. RAB0071, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 15 pg/mL standard curve range: 24.69-18000 pg/mL |
| detection method | colorimetric |
| input | sample type serum sample type plasma sample type cell culture supernatant(s) |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | CCL27 (C-C motif chemokine ligand 27) contributes to the progression of inflammatory skin diseases. In animal models, atopic dermatitis is known to increase the CCL27 gene expression. The CCL27 gene is downregulated in cancer. It regulates the enrollment of T cells to inflamed skin. |
| General description: | The Mouse CTACK ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CTACK in serum, plasma, cell culture supernatants and urine. The CCL27 (C-C motif chemokine ligand 27) gene codes for a T cell skin-homing chemokine and is expressed in epidermal keratinocytes. It is also referred to as cutaneous T cell-attracting chemokine (CTACK). |
| Immunogen: | Recombinant Mouse CTACK |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 1X Assay/Sample Diluent Buffer A (Item D1); ELISA 5X Assay/Sample Diluent Buffer B (Item E1); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |