Human Granulocye-Macrophage Colony Stimulating Factor / CSF2 ELISA Kit
SIGMA/RAB0100 - for serum, plasma, cell culture supernatant and urine
Synonym: GM-CSF
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human Granulocye-?Macrophage Colony Stimulating Factor / CSF2 ELISA Kit, Cat. No. RAB0100. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human Granulocye-?Macrophage Colony Stimulating Factor / CSF2 ELISA Kit, Cat. No. RAB0100, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 2 pg/mL standard curve range: 2.05-500 pg/mL |
| detection method | colorimetric |
| input | sample type serum sample type urine sample type plasma sample type cell culture supernatant(s) |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | GM-CSF (granulocyte-macrophage colony-stimulating factor, also referred to as CSF2) is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It enhances neutrophil migration. GM-CSF plays a crucial role in inflammatory and autoimmune diseases. In neutrophils, it promotes the expression of TLR2 (toll-like receptor) and TLR2-associated interleukin 8 responses, thereby eliminating microbial attack. |
| General description: | The Human GM-CSF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GM-CSF in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human GM-CSF |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Assay/Sample Diluent Buffer E (Item E2); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |