Human Cystatin C ELISA Kit
SIGMA/RAB0105 - for serum, plasma, cell culture supernatant and urine
Synonym: Cystatin-3; Cystatin-C
Product Type: Chemical
Standard Curve : Typical Standard Curve for Human Cystatin C ELISA Kit, Cat. No. RAB0105. The standard curve(s) displayed are for demonstration only. A standard curve must be run with each assay.
Preparation of Standard : Preparation of Standard for Human CystatinC ELISA Kit, Cat. No. RAB0105, showing dilution factor.
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 300 pg/mL standard curve range: 0.313-20 ng/mL |
| detection method | colorimetric |
| input | sample type plasma sample type urine sample type serum sample type cell culture supernatant(s) |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| ELISA: suitable |
| Application: | For research use only. Not for use in diagnostic procedures. Please refer to the attached General ELISA KIT Procedure  (sandwich, competitive & Indirect ELISA) |
| Biochem/physiol Actions: | Cystatin C (CysC) is released by all nucleated cells. Abnormal thyroid function, immunosuppressive therapy and systemic inflammation alter the levels of CysC. Mutation in the gene encoding CysC causes hereditary cystatin C amyloid angiopathy, which is a consequence of amyloid deposits containing the CysC variant in the brain. |
| Biochem/physiol Actions: | Cystatin-C precursor (CST3) is an inhibitor of lysosomal cysteine proteases and several cathepsins like cathepsin S, a lysosomal enzyme. It has been shown that CST3 is a marker of glomerular filtration and diabetic kidney disease. In the presence of pathological conditions, it forms amyloid fibrils in brain arteries. Mutations in the gene encoding CST3 have been shown to be associated with a form of amyloidosis and age-related macular degeneration. |
| General description: | The Human Cystatin C ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human cystatin C in serum, plasma, cell culture supernatants and urine. |
| Immunogen: | Recombinant Human CystatinC |
| Other Notes: | A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| RIDADR | UN 3264 8 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | ELISA 5X Assay/Sample Diluent Buffer E (Item E2); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B) |